The Goal Decision System will be on standby for the first time in the fifth-round games at venues where the technology is currently installed.
Subsequent matches in the semi-finals and finals next year will also have the system at their disposal.
Following testing over the past two weeks, FIFA has now confirmed the Mitre Delta V12 ball - the official matchball of the Capital One Cup - is now calibrated to be used with Hawk-Eye's ground-breaking system.
The Goal Decision System has been used at all Premier League matches this season and has also been installed at Wembley Stadium.
Andy Williamson, the Football League chief operating officer said: "The Football League is a long-term supporter of the use of goal-line technology even if its cost currently puts it beyond the reach of our own clubs.
"In many ways its use in cup football is even more important than in league football, as any decision about whether the ball has crossed the goal-line can mean the difference between a club being knocked out or not, and could even determine the eventual Wembley winners."
Check out if the GDS system is required in the Sunderland v Chelsea tie live on Sky Sports 1 from 7.30pm on Tuesday