Hull arrive having given some of Spurs' title rivals a run for their money already this season, and they will have former Spurs star Tom Huddlestone in the centre of their midfield.
The 26-year-old spent eight seasons at White Hart Lane but chose to leave in the summer in search of first-team football.
"We're all looking forward to seeing him," Defoe said of Huddlestone, who could play Spurs again in Hull's Capital One Cup tie in midweek.
"He was a great player here and is doing well for Hull, pulling strings and stuff. It is going to be a tough game but it is a game we have to win.
"It is important we keep winning football matches. I am looking forward to both games. We're a club that is going places."
Defoe equalled Martin Chivers' Spurs record of 22 goals in Europe when he scored in the 2-0 win over Sheriff Tiraspol in the Europa League on Thursday night.
He is finding it hard to break into boss Andre Villas-Boas' first team in the Premier League but he is proud to match Chivers' mark.
"It is special because he is a legend at the club, someone I look up to and respect. To be up there is great," he said.
"One more goal I will be on the top on my own, and to have someone around you like Martin Chivers is special."