The Tigers had performed well in stifling Spurs' attacking threat for much of the game before referee Michael Oliver gave a spot-kick to the hosts 10 minutes from time which was duly converted by Roberto Soldado.
The controversial call came after Jan Vertonghen's cross was adjudged to have been handled by Ahmed Elmohamady, but Bruce was left fuming at the call.
"It is an absolute joke decision," he said. "It hits his leg for a start and then goes up and hits his arm, how do you give a penalty in that situation?
"We have more directives and meetings and all this nonsense, they are all getting assessed up in the stands but then they give decisions like that which just baffles me and I have to ask the question, would we have got it?
"The disappointment I have is that I have lads playing today who are desperate to play in the Premier League.
"They are desperate to make a fist of it and they are now questioning themselves after Everton last week, where we got some decisions against us and today where, in my opinion, he couldn't wait to give it."
Bruce was seen remonstrating with Oliver at full-time but admitted any argument was futile and revealed Spurs boss Andre Villas-Boas acknowledged it was a tough decision on Hull.
When asked if he had spoken to Oliver, Bruce replied: "After the game, but what difference does it make?
"It is the same old thing. He's ballsed it up. If my player made a decision like that I wouldn't be playing him next week and if I kept making decisions like that I would get the sack.
"He [Villas-Boas] absolutely, totally agreed. He is going to go and see it and know it is a soft one and in my opinion it is not right, not for the stakes we are playing for - a big game, at White Hart Lane and he has robbed the players of a well-earned point.
"We could get relegated by a point, you never know it is too early yet, but it is what it is."