Messi was taken off on a cart late in the Champions League match, having collided with goalkeeper Artur Moraes and then twisted awkwardly when trying to curl a shot into the net in the 84th minute.
In an article on their official website, Barca said further tests would be required before a timescale could be put on any absence for the Argentinian forward.
He had only come on as a 58th minute substitute with Barcelona already assured of their progress from Group G.
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