Sevilla defender Alberto Moreno is the new face in the 23-man Spain squad that will face Belarus and Georgia in next week's World Cup qualifiers - but there is no place for either Diego Costa or Roberto Soldado.
Twenty-one-year-old left-back Moreno graduates from the Under-21s after making a solid start to the Primera Division season with Andalusian club Sevilla.
Coach Vicente del Bosque has, however, been unable to call up Brazilian striker Costa, who last month switched his allegiance to Spain, due to outstanding paperwork but winger Juan Mata is included despite struggling to hold down a place in the Chelsea team under Jose Mourinho.
Tottenham striker Soldado, who has scored four goals for the London club this term, is omitted but Manchester City stars Jesus Navas, Alvaro Negredo and David Silva will join their Spanish team-mates at a training camp ahead of the Group I double-header. Left-back Nacho Monreal represents Arsenal.
Reigning La Liga champions Barcelona have seven representatives - including Victor Valdes, Xavi and Andres Iniesta - while Iker Casillas and Isco are among four players called from Real Madrid.
Santi Cazorla, Jordi Alba, Carles Puyol, Thiago Alcantara, Fernando Torres and Javi Martinez are the most notable injury absentees as the world champions look to secure their place at next summer's Brazil finals by claiming four points from their two remaining games.
Spain are tied on 14 points for first place with France, but Les Bleus have only one game left to play - against Finland on October 15.
Belarus are first up for Del Bosque's men in Palma on October 11 before La Roja host Georgia in Albacete four days later.
The Spain coach told a press conference: "Alberto Moreno is a dynamic player, can perform various different functions and has played some great games recently.
"Diego Costa made a sporting decision and we had considered him but FIFA intervened. He's not a typical number nine, he offers something different and has been developing his game in Spain for a long time."
Costa has represented Brazil in two friendly matches this year but the South American nation's football federation has been asked to provide a certificate confirming he has not played in an "official game".
Spain squad: Casillas (Real Madrid), Reina (Napoli), Valdes (Barcelona); Arbeloa (Real Madrid), Juanfran (Atletico Madrid), Pique (Barcelona), Albiol (Napoli), Ramos (Real Madrid), Monreal (Arsenal), Alberto Moreno (Sevilla); Busquets (Barcelona), Suarez (Atletico Madrid), Xavi (Barcelona), Iniesta (Barcelona), Koke (Atletico Madrid), Isco (Real Madrid), Pedro (Barcelona), Navas (Manchester City), Fabregas (Barcelona); Negredo (Manchester City), Mata (Chelsea), Silva (Manchester City), Villa (Atletico Madrid).