The 24-year-old limped off during the second-half of Saturday's win over Carlisle and Wilson believes the game was tempered by a heavy challenge on the Blades midfielder.
"Kev was on the end of a really nasty challenge. He strapped it up for the second half but it was soon obvious he couldn't continue," he told the club's official website.
"We don't know how bad it is because of the swelling. We will access him in due course, but looking at the state of his ankle it doesn't look too clever."
Wilson was disappointed to see Graham Salisbury only show a yellow card to former United striker Danny Cadamarteri over the incident.
"It was a very late and high tackle and it is frustrating that challenge is punished the same way as a shirt pull," he added.