The third-placed Bees will meet second-placed Rovers at Griffin Park to decide the final promotion spot in League One, with Rosler's side two points adrift after their 1-1 draw at already-relegated Hartlepool.
Victory is a must in the game, and Rosler admitted: "We have worked so hard in the last few months to be in the position where we have it in our own hands and now it all leads to the final day.
"It's a home game and we have enjoyed a great achievement in getting there - Doncaster have everything to lose and we have everything to gain."
He continued: "This wasn't the greatest game by any means, in the 25 minutes until they scored we were not at the races, then we lose our nerves.
"When we started to attack we scored and then in the second half we were the better team and had some very good chances.
"Tom Adeyemi had two chances to win the game, we know we can all play better, no question about it.
"Credit to Hartlepool, they showed character and didn't drop their heads after relegation.
"But we are where we want to be and are full of optimism - now we look forward to a full house at Griffin Park."