The Bees host the second-placed Rovers knowing a victory will see them leapfrog their opponents and secure Championship football for the first time since 1993.
Rosler knows the pressure will be on both sides but has backed his players to rise to the occasion.
"I like that we have a week to prepare," he said. "Our best performances have come when we have a week to build up to a game.
"We want to build it up. This is something to look forward to. It is positive pressure, these are the games we want to be involved in.
"This was the clear message I had since June 2011 when I took over. Now we are there.
"Let's build it up, create the excitement, that will only help us."