Town, only promoted to the Football League in 2011, head to a Wolves side who were playing in the Premier League two years ago.
"We had some big games last season, including trips to places like Sheffield United and Doncaster where we acquitted ourselves really well," said Barker.
"But this is another step up. Two years ago Wolves were in the Premier League and we were finding our feet in League Two. If someone had said that two years on the teams would be playing each other in a league game you might have got some funny looks.
"It's a massive occasion for the football club and I'm sure our fans are looking forward to going to a stadium like Molineux which is geared up for the Premier League.
"But we absolutely want to go there and put in a performance."