Yussuf celebrated his return to action with a goal after coming on as a substitute in Tuesday's reserve match against Tranmere. But five minutes later, the 20-year-old suffered a recurrence of the groin injury that has kept him out of the first-team squad since mid-October.
Albion are waiting to discover the extent of the problem but the former Leicester trainee is set for an extended spell on the sidelines.
Some good news for manager Gary Rowett saw Aaron Webster play the full 90 minutes in the 2-2 draw in his first run-out since suffering a hamstring injury in training more than a month ago.
Rowett also gave loan signing Jacob Blyth his first full outing. The young Leicester striker had previously made brief appearances from the bench in the League Two defeats against Aldershot and Wycombe.
