It was the Chairboys' first defeat since New Year's Day, but they sit a healthy 12 points clear of the relegation zone.
Although disappointed to concede so early from a set-piece, Ainsworth vowed to rectify such mistakes on the training pitch and has faith his side will respond against Bristol Rovers.
"I've always said we're not going to win every game and sometimes you take positives out of defeat and this has brought us down to earth and made sure we will be on it at the weekend," said Ainsworth.
"We were done by a set-play in the first minute and I thought after that we were chasing the game and it's really important to defend early on and not let a team get a lead.
"I told the lads don't be down because we've been on a great run and what they have achieved in the last two and a half months, from 24th to 12th in the league, is something to be proud of.
"The main thing is do we still believe we can beat Bristol Rovers on Saturday. The last time we got a hammering the lads responded really well and I still believe we can beat anyone."