Watford loanee Britt Assombalonga's late winner and Barry Corr's tap in just after half-time completed a comeback win for Southend, their first in four in the league.
Dean Morgan had given Wycombe hope of ending their own run of back-to-back defeats after pouncing in the seventh minute, but it wasn't to be for the Chairboys, and Ainsworth admitted he would have to reconsider his team selection.
"We have to look at something. Maybe people who might have been waiting for an opportunity will get an opportunity," he said.
"I wasn't happy with the second-half performance and I told them that. I'm not one to go into the dressing room and say well done.
"We played the way we wanted to. We gave away two really poor goals. That's the annoying thing. Both goals were avoidable.
"We created some chances - more than we have done in recent weeks - but we have to pick ourselves up for our next game.
"We had some great chances; I'm pleased that we are creating things that we can put away. It is just a case of sticking them away now.
"There are a few things that we can work on but that is football."