Wootton twisted his ankle on a rain-sodden pitch at Charlton on 9 November and will definitely be out of this weekend's clash against Middlesbrough.
However, Leeds boss Brian McDermott is confident that defender Marius Zaliukas will be an adequate replacement, with the Lithuanian likely to make his full Leeds debut on Saturday.
Speaking about Wootton's injury, McDermott told the Yorkshire Evening Post: "I don't think the pitch helped and he won't be ready for Saturday.
"He'll be out for a while but I'm not really able to put a timescale on it. We're lucky that we've got Marius ready to come in."
Leeds have been boosted by the news that right-back Sam Byram is in contention for the match against Boro, but his manager has warned him that he is not an automatic starter following Lee Peltier's impressive performances against Yeovil and Charlton.
"Sam's training and he's fine, but Lee's been excellent for us and this is why you have a squad," McDermott added.
"When one good player comes out, another good player has to come in.
"I've got a decision to make and for me it'll be a case of picking the best team for the strengths and weaknesses Middlesbrough have."