On Thursday Appleton confirmed an initial bid from Liverpool had fallen short of the club's valuation of the England Under-21 winger, who left Anfield last summer having made one senior appearance.
Asked after the game whether Liverpool had upped their bid, Appleton said: "No, not that I'm aware of."
Ince again impressed at Bloomfield Road but was left frustrated as goalkeeper Carl Ikeme produced a series of fine saves to help Wolves to a 2-1 victory.
On Ince's impressive individual display, he added: "It was important for him and he's got to show a strength of character now to suggest that, with everything going on, all he wants to do is play football and play well for Blackpool Football Club."
Appleton continued: "You don't have to be a rocket scientist to weigh up the whole game and look at the opportunities we created in both halves and say we probably should have got something out of the game.
"But it wasn't to be, a poor two or three minutes at the start of the game and we won't talk about the other decision.
"I think he's given it for pulling of the shirt, but if it was a pull of the shirt it was the slightest of pulls and definitely he was outside the box, quite comfortably. It's disappointing."