Chelsea have submitted two unsuccessful bids for Rooney, who missed Sunday's FA Community Shield win over Wigan with a shoulder injury.
Moyes was in a combative mood at the post-match press conference at Wembley, where he denied speculation that he had fallen out with Rooney.
"We don't want to sell Wayne," Moyes responded when it was suggested the 27-year-old wanted to leave Old Trafford for Stamford Bridge and had been told to train with United's reserve team.
"I have not fallen out with Wayne and the reason he trained with the reserves was because he wanted to because he wasn't allowed any contact."
Despite the injury, Rooney has been included in England's squad for their meeting with Scotland on Wednesday, something Moyes said he was happy about.
"I want him to join up (with the England squad)," added Moyes.
"He trained with us yesterday (Saturday). He's not ready, you'll probably see that in the game. He'll probably play a chunk of the game.
"He's had no football. He's had 45 minutes against Real Betis in a friendly when he hurt his shoulder. Apart from that, since the day he got back for pre-season, he's got no match action.
"So you probably wouldn't expect somebody in that condition to be playing for a long period."
United failed in their bid to sign Cesc Fabregas from Barcelona, but Moyes admitted that adding new players to his squad is still on his agenda.
He continued: "I hope so, we move on every day and the transfer window gets closer and closer. But if we have to start the season with what we've got then I'll be happy."