Eardley, who arrived at Bloomfield Road last week from Oldham in a £350,000 transfer, was in the Wales squad for Wednesday's friendly in Montenegro.
His availability is a boost to Holloway as his men go in search of their first Championship win of the campaign.
Holloway made wholesale changes for Tuesday's 2-1 win at Crewe in the Carling Cup as Matt Gilks was given a run-out in goal, while new recruits Mark Hudson and Adam Nowland were also handed starting roles.
First-choice stopper Paul Rachubka is expected to return between the posts and midfielder Charlie Adam should also come back into the starting line-up.
Striker Ben Burgess is hoping to shake off a minor knee injury but defender Danny Coid is definitely sidelined with a hairline fracture on his tibia.