Rovers endured a dismal start to the season but four points from their last two outings have breathed new life into their campaign.
Barnes has reported no new injury concerns to his squad and will be reluctant to make changes after Tuesday's morale-boosting 1-0 win at Wycombe.
Striker Michael Ricketts felt his groin in the warm-up at Adams Park but went on to play the full 90 minutes without any problems.
He should again lead the line but midfielder Alan Mahon looks set to be on the bench again.
Barnes said: "I know Kenny Jackett from our Watford days and his teams are always well organised, so that's what we expect Millwall to be."