Hughes should have originally been available for the clash at The Den but the Boxing Day game with Walsall fell victim to the weather, so that means his one-match ban carries over.
Ben Swallow is likely to replace him, although Dominic Blizzard may also come in having played on the left wing this season.
Jo Kuffour could be a beneficiary of Saturday's postponement though, as he was struggling with an ankle injury, but the extra recovery time means he could be in contention.
With Chris Dickson having return to Charlton after an impressive loan spell, Daryl Duffy is set for a start in attack and he will be keen to impress with boss Paul Trollope looking to strengthen his front line.
The Pirates have lost their last six away games and Trollope is keen to address that poor form, telling the club's official website: "There have been a number of close away games on the road of late and things may have been different. But the form and the results have not been good enough on the road."