Holt, who has scored 30 goals since joining from Shrewsbury last summer, has sat out the last two games through suspension but is back and gunning for a club record.
Holt needs seven more goals to equal Terry Allcock's record from 1963 and has three games remaining.
Midfielder Korey Smith missed last weekend's 1-0 win at Charlton due to an ankle knock while manager Paul Lambert is waiting on the fitness of midfielder Wes Hoolahan.
The Irishman has sat out the last four games with a thigh injury but is unlikely to return.
Lambert and head of football operations Gary Karsa will watch the game from the stands after receiving one-game touchline bans by the Football Association Regulatory Commission for their conduct during the match against Tranmere on April 2.