O'Neill conceded Villa have "a couple of problems" ahead of the meeting with Roberto Mancini's side but did not go into any specific details.
Central defender Richard Dunne played in the derby win over Birmingham with his head bandaged after an injury suffered against Hull, and will be keen to battle on against the club he left to join Villa for £6million in the summer after nine years' service.
O'Neill said: "There are just a couple of problems. We still have another training session to negotiate but overall we are not too bad."
Villa (from): Friedel, Cuellar, Dunne, Collins, Warnock, Downing, Milner, Petrov, Ashley Young, Agbonlahor, Carew, Guzan, Luke Young, Sidwell, Delfouneso, Heskey, Reo-Coker, Beye.