The duo made their comebacks from injury as substitutes against Fulham on Sunday and are pressing to start the third-round game.
England Under-21 international Kightly had been sidelined since March by hip and foot problems, while Ebanks-Blake strained his hamstring on the opening day of the season against West Ham.
Wolves boss Mick McCarthy could also give a first start to striker Stefan Maierhofer following his recent £1.8million move from Rapid Vienna.
Kick-off is at 8pm.
Wolves (from): Hennessey, Hahnemann, Ikeme, Foley, Berra, Zubar, Craddock, Elokobi, Hill, Kightly, Halford, Henry, Milijas, Castillo, Jones, Edwards, Jarvis, Ebanks-Blake, Maierhofer, Keogh, Vokes, Doyle.
