Both players missed the weekend win over Colchester through injury and face a race against the clock to prove their fitness. Defender Kisnorbo is struggling with an Achilles/calf injury and midfielder Kilkenny has an ankle problem.
Leeds will also be without winger Max Gradel and new loan signings Gary McSheffrey and Shane Lowry, who are all ineligible for the fourth-round replay.
But manager Simon Grayson could have Andrew Hughes available following illness and he could step back in at left-back for Aston Villa youngster Lowry, while Bradley Johnson, who made way for McSheffrey against Colchester, could also return.
Lubo Michalik is expected to partner Richard Naylor in central defence should Kisnorbo fail to make it, with Grayson to decide which combination to go with.