Leeds manager Simon Grayson is expected to name an unchanged side for the third game running against the Tykes.
Grayson has no new injury or suspension problems following Saturday's 2-2 draw with Norwich as his promotion-chasing side look to make it 20 league games with just one defeat.
Grayson said: "We have a few knocks here and there, but that's not surprising given how Saturday's game panned out.
"The lads did their recovery on Saturday and were back in on Sunday morning preparing for this game."
Striker Davide Somma is pushing for a return to the starting line-up after stepping off the bench to volley the equaliser with his first touch. It was the South African's 12th goal of the season.
Leeds, in sixth place, could go level on points with second-placed Nottingham Forest if other results go their way.
Barnsley will be without midfielder Hugo Colace for their midweek clash at Yorkshire rivals Leeds.
The Argentinian, current player of the year at Oakwell, will be sidelined for four weeks after tearing an abductor muscle in training.
Colace, who missed Saturday's defeat at Portsmouth, is one of four players ruled out of action.
Skipper Stephen Foster has a "leg muscle problem" sustained in the recent draw with Ipswich and fellow defenders Jay McEveley and Luke Potter are both working their way back from serious knee injuries.
Otherwise, Tykes boss Mark Robins has no new injury or suspension problems and is expected to retain Paul McShane in his back four following the Republic of Ireland international's debut on Saturday.
Strikers Garry O'Connor and Andy Gray and midfielder Goran Lovre are all pushing for recalls.