Mark Robins is waiting on the fitness of Nathan Doyle and Bobby Hassell ahead of the game.
Midfielder Doyle missed Saturday's goalless draw at Derby due to a calf injury, while defender Hassell was sidelined with a hamstring strain.
The Tykes will also be without on-loan striker Frank Nouble, who has returned to parent club West Ham.
Defender Jay McEveley was back in action for the first time in nearly five months against his former club on Saturday at Pride Park following a knee injury, but two games in four days could be too much and he might be rested.
Teenage striker Steven Bennett made only his third appearance for the club when stepping off the bench for Garry O'Connor on Saturday and will be hoping to play a bigger part against Scunthorpe as the Tykes chase their first win in seven matches.
Ian Baraclough will be reluctant to make changes when his side travel to Barnsley.
The Iron beat high-flying Swansea 1-0 on Saturday to reignite their survival bid and Baraclough was delighted with his players' performance.
He took a risk by leaving winger Mark Duffy on the bench, but it proved to be a tactical masterstroke as Scott Sinclair and Nathan Dyer struggled to get in behind the Scunthorpe defence.
However, Baraclough will again be missing a number of players, with defender Paul Reid heading the list of absentees due to a hip injury.
Striker Chris Dagnall (knee), goalkeeper Joe Murphy (ankle) and right-back Cliff Byrne (back) could all miss out again.
Yet Baraclough said: "Momentum is with us and I think the belief is coming that we can beat anybody on our day if we do things right."