Adam Lallana is expected to return to Saints' starting line-up.
Saints' star midfielder played the final 17 minutes of Tuesday night's 2-0 defeat at Rochdale to prove his fitness after a groin injury.
Defender Dan Harding looks set to miss out after limping off with a groin problem just before half-time.
Ryan Dickson should therefore revert to left-back leaving a spot in the midfield for Lallana.
Richard Chaplow will return to the squad after he was rested in midweek but fellow midfielder Morgan Schneiderlin (knee) and winger Alex Chamberlain (ankle) will not be risked.
Saints remain in the second automatic promotion place despite falling to their first defeat in nine matches at Spotland.
Rovers are sweating on the fitness of top-scorer Will Hoskins.
The 19-goal striker injured his ankle in the 1-0 win over Bournemouth and missed last weekend's loss to Exeter.
But Hoskins will train on Friday and caretaker manager Stuart Campbell will leave it late until deciding whether to risk his frontman.
Danny Senda took a knock to his knee against the Grecians but has recovered and should play, while Byron Anthony has shaken off an illness that has laid him low recently.
Rene Howe has recovered from tonsilitis and could be involved as Campbell has no other selection concerns to contend with.
The Pirates will be grateful for anything they can pick up at St Mary's as they remain in deep relegation trouble, sitting in the bottom four with five games to play.