Scunthorpe boss Alan Knill will have Joe Garner back for the vital clash.
The striker served a one-match ban during the Iron's 1-1 draw at Coventry which kept their survival hopes alive.
Garner was replaced by Bobby Grant at the Ricoh Arena and Knill must now decide whether to stick with Grant or bring Garner straight back in.
Andy Hughes managed 90 minutes on Good Friday despite struggling with a groin problem and he should be fine again for the Glanford Park clash.
Michael O'Connor was only on the bench after recovering from a thigh injury and he may be in line to start.
Defender Cliff Byrne and midfielder Garry Thompson remain sidelined.
Millwall skipper Paul Robinson is expected to return to the starting line-up.
The Lions defender sustained a rib injury in the warm-up before the recent defeat at Coventry and was an unused substitute during Saturday's home win over Preston.
Darren Purse stepped into the starting line-up against North End and scored his first goal for the club.
Manager Kenny Jackett will still be without his three senior strikers as Steve Morison and Neil Harris are suspended while Kevin Lisbie is still struggling with an ankle injury.
Josh McQuoid struck a stunning first goal for the club on Friday and strike partner John Marquis scored his second goal in only his second start, so Jackett will have no qualms about retaining the pair at Coventry.
Winger Hameur Bouazza stepped off the bench on Saturday in place of McQuoid and gives Jackett an extra option as his side look to close the two-point gap between themselves and the last play-off spot.