Little is beginning a six-week lay-off after injuring his knee against Huddersfield last week and the former Wolves full-back will go under the knife in the coming days.
Tommy Rowe came into the side in his place for the Carling Cup win over Cardiff in midweek and is likely to play again as Gary Johnson's side look to build on fine back-to-back victories.
Centre-half Gabriel Zakuani played for an hour in the reserves this week and is likely to be available for selection after his groin injury but midfielder Lee Frecklington (knee) and winger Chris Whelpdale (thigh) remain on the sidelines.
