Tranmere boss Les Parry will be without Jermaine Grandison for the clash against Brighton.
The young defender picked up an injury just before half-time during the 1-0 win over Bristol Rovers and has since returned to his parent club Coventry for treatment, cutting short his loan deal at Prenton Park.
Nick Wood is likely to fill the void following his solid second-half performance in midweek, while on-loan Derby man Arnaud Mendy is set to continue in midfield after extending his loan spell by another two months.
But Parry will continue to be without the likes of Gunnar Nielsen, Marlon Broomes, Ash Taylor, Liam Darville and Alan Mahon, who are yet to recover from injuries.
Leaders Brighton will be without defender Adam El-Abd, who collected his fifth yellow card of the season against Brentford on Tuesday night and sits out with a one-match ban.
His absence could pave the way for Gordon Greer to finally cement his place in the starting line-up.
The centre-half was named Seagulls captain at the start of the season after joining from Swindon but two suspensions and a calf problem have restricted him to less than an hour of first-team action so far.
However, Greer is back in full training and will vie with youngster Lewis Dunk to start alongside Tommy Elphick.
Winger Kazenga LuaLua, who scored the winner in midweek, will have his hamstring problem checked over while midfielder Alan Navarro (knee) is still sidelined.