Tranmere boss Les Parry could be without strikers Enoch Showunmi and Ian Thomas-Moore for the Johnstone's Paint Trophy clash at home to Huddersfield.
Showunmi sustained a dead leg and Thomas-Moore picked up a calf injury during Saturday's 2-1 home defeat to Leyton Orient and are rated as doubtful.
Parry said: "It turns out Enoch's was a dead leg but it is just above the knee. Ian Thomas-Moore's calf is more of a concern."
Several of Rovers' injured players are approaching full fitness again but the clash against Lee Clark's side may come too soon for them.
Parry added: "We've got to gamble with some of the subs who will be on the bench simply because they haven't played so long."
Huddersfield manager Lee Clark is without defender Peter Clarke.
Clarke received his fifth booking of the season in the 2-1 win over Brighton at the weekend and serves a one-match ban. Nathan Clarke or Liam Ridehalgh could deputise at centre-half while former Tranmere man Antony Kay is another option.
Clark could also be without left-back Graham Carey and midfielder Damien Johnson, who picked up knocks in an aggressive encounter against the leaders on Saturday.
Arsenal loanee Benik Afobe is unavailable for the trip after the striker returned to his parent club for an FA Youth Cup tie.
Defenders Gary Naysmith and Tom Clarke returned to training last week and could be added to the squad while fit-again striker Lee Novak is likely to occupy the bench again.
