Arsenal will hand striker Robin van Persie (knee) a fitness test on Tuesday morning.
Captain Cesc Fabregas has recovered from a hamstring problem and should face his old club.
However, midfielder Alex Song (knee) and England winger Theo Walcott (ankle) remain sidelined.
Barcelona will be unable to field either of their first-choice central defenders as captain Carles Puyol has failed to recover from a knee injury while Gerard Pique is suspended.
Sergio Busquets is expected to drop into central defence alongside Eric Abidal.
Arsenal (from): Almunia, Szczesny, Djourou, Clichy, Eboue, Gibbs, Koscielny, Sagna, Squillaci, Fabregas, Arshavin, Wilshere, Rosicky, Nasri, Diaby, Denilson, Bendtner, Chamakh, van Persie.
Barcelona (from): Valdes, Pinto, Mino, Dani Alves, Milito, Maxwell, Abidal, Fontas, Xavi, Iniesta, Mascherano, Keita, Busquets, Adriano, Vazquez, Thiago Alcantara, Villa, Bojan, Messi, Pedro, Jeffren, Afellay.