Tranmere boss Les Parry is set to be without youngster Dale Jennings again.
The highly-rated striker is still troubled by a groin injury and is unlikely to play any part against Gary Megson's men.
Ash Taylor and Ian Goodison are set to be passed fit despite picking up minor knocks in Saturday's 3-1 win at Plymouth.
Taylor sustained a slight jaw injury and Goodison picked up a hamstring strain but the complaints are not serious and will not prevent them facing the Owls.
However, midfielder Ryan Fraughan has damaged medial ligaments in his knee and will be out for around six weeks.
Saturday's victory at Home Park lifted Rovers three points clear of the relegation zone.
Sheffield Wednesday defender Lewis Buxton has been ruled out against Tranmere due to a hamstring strain.
Buxton has been struggling with the problem in recent weeks and aggravated it during Saturday's 2-1 defeat at Rochdale, so Richard Hinds, Reda Johnson and Tommy Spurr are all hoping to be in contention for a return to the side. Another defender, Jon Otsemobor, remains sidelined with a calf injury.
Midfielder Isaiah Osbourne, on loan from Aston Villa, is pushing for his first start after stepping off the substitutes' bench on Saturday, as is striker Gary Madine, who scored his third goal in four appearances for the Owls in the closing stages.
Manager Gary Megson is chasing his first win in charge, while the Owls are bidding for their first win in nine league matches.