Bolton manager Owen Coyle has indicated he will freshen things up for the cup date with their neighbours.
He could hand a debut to former Middlesbrough defender David Wheater while striker Ivan Klasnic may come into contention after a groin injury.
Goalkeeper Adam Bogdan is set to be recalled to the cup team after his fine display in the third-round victory against non-league York, and that applies to Spanish forward Rodrigo Moreno as well.
Wigan manager Roberto Martinez will give his fringe players another chance to shine.
The Spaniard played a much-changed side for the third-round win over Hull and will do the same at the Reebok Stadium, with striker Callum McManaman set to be given another chance along with fellow youngsters Jordan Mustoe, Jordan Robinson and Daniel Redmond.
James McCarthy and Franco Di Santo will be given a weekend off after their recent returns from injury but Gary Caldwell is available again after suspension.
Bolton (from): Bogdan, Jaaskelainen, Robinson, Cahill, Knight, Wheater, Muamba, Petrov, K Davies, Elmander, Klasnic, Taylor, Ricketts, Alonso, Moreno, M Davies, Cohen, Holden, Steinsson, Blake, O'Halloran.
Wigan (from): Al Habsi, Pollitt, Kirkland, Gohouri, Stam, Figueroa, Watson, Diame, McArthur, Thomas, N'Zogbia, McManaman, Rodallega, Lopez, Gomez, S Caldwell, G Caldwell, Boyce, De Ridder, Golobart, Mustoe, Robinson, Redmond.