Stevenage boss Graham Westley will be without Byron Harrison.
The striker, who scored just three minutes after coming on as a substitute in the 3-0 midweek win over Rotherham, is cup-tied having already played in this season's competition for Carshalton Town.
Midfielder Stacy Long and striker Yemi Odubade are again doubtful after missing out against the Millers with calf and back injuries respectively, while midfield duo Darren Murphy and Joel Byrom are still sidelined with shoulder and foot/ankle problems respectively.
Boro will be hoping to cause another FA Cup upset after dumping Premier League side Newcastle out at the third round stage.
Reading have injury doubts over defender Alex Pearce and midfielder Brian Howard.
Both players will be monitored ahead of the trip, where Premier League side Newcastle succumbed in the previous round.
Noel Hunt may be available again having missed last week's 1-1 draw at home to Hull while fellow striker Shane Long should also be fine despite receiving lengthy treatment in the same match.
Midfielder Michail Antonio is expected to be in contention despite suffering from a back problem which ruled him out against the Tigers.
However, new signings Mathieu Manset, Brett Williams and Sean Morrison are all cup-tied.
The Royals made it to round four courtesy of a 1-0 win over West Brom at the Madejski Stadium.