Villa's record signing, Darren Bent, is cup-tied.
Bent, signed from Sunderland for £18million, played and scored for the Black Cats when beaten by Notts County in the previous round.
With John Carew having joined Stoke on loan, Emile Heskey is set to return to the starting line-up.
Ciaran Clark (hamstring), Fabian Delph (knee) and Kyle Walker (back) are all available again.
But Luke Young and Stephen Ireland are both still sidelined with knee injuries.
Ryan Nelsen will be missing for Blackburn with a knee problem.
Three midfielders are back from long injury absences however - Steven Nzonzi could start while Keith Andrews and Vince Grella will also be part of the squad.
El-Hadji Diouf will be aiming for a recall after being on the bench against West Brom last time out.
Villa (from): Friedel, Walker, Collins, Dunne, Cuellar, Clark, Baker, Albrighton, Petrov, Makoun, Pires, Reo-Coker, Delph, Downing, Bannan, A Young, Marshall, Agbonlahor, Heskey, Delfouneso, Lichaj.
Blackburn (from): Robinson, Samba, Olsson, Salgado, Givet, Dunn, Pedersen, Hoilett, Kalinic, MB Diouf, Lowe, Roberts, Santa Cruz, E-H Diouf, Benjani, Nzonzi, Hanley, Andrews, Grella, J Jones, Bunn, Brown.