Gareth Bale looks set to start for Tottenham.
The Wales winger trained with his team-mates at the Bernabeu and will be in manager Harry Redknapp's line-up if he suffers no recurrence of the hamstring injury which has troubled him recently.
William Gallas could make a surprise start just three days after Redknapp said a knee injury meant he could not kick a ball, but Niko Kranjcar is a doubt with a foot problem and Steven Pienaar did not travel due to a groin injury.
Gallas' return would be welcome given that fellow defenders Ledley King (groin), Jonathan Woodgate (calf), Younes Kaboul (thigh) and Alan Hutton (knee) are all unavailable.
Redknapp said: "Hopefully Gareth will be fit. He is going to train tonight. Let's hope he has no problems and if he is OK then he will play.
"William Gallas is going to train tonight. Hopefully he will be fit. Steven Pienaar didn't travel. He has a groin injury.
"Niko Kranjcar is struggling with a foot injury. He kicked the bottom of someone's boot on Saturday against Wigan. It is badly bruised and swollen. In fact at the moment we might have six subs on the bench instead of seven."
Cristiano Ronaldo has been included by Real Madrid.
Ronaldo had been a doubt for the match after suffering a hamstring injury against Atletico Madrid on March 19 that ruled him out of Portugal's two international friendlies and Saturday's Primera Division defeat to Sporting Gijon.
However, after the Sporting match, manager Jose Mourinho hinted that he might take a risk on Ronaldo to face Spurs and, after Monday afternoon's training session, included the world's most expensive player in a 19-man squad.
Also named in Mourinho's squad were Brazilian duo Marcelo and Kaka, as well as Argentina winger Angel Di Maria, who had all been fitness doubts along with Ronaldo.
Full-back Marcelo picked up a rib injury while on international duty, while Kaka has been absent since late February with a knee injury and Di Maria suffered a knock against Sporting.
Argentina striker Gonzalo Higuain is also included again having made his return to action against Sporting after over four months out with a back problem that required surgery.
However, in-form forward Karim Benzema misses out through injury, as confirmed by Mourinho earlier today.
Benzema, who has scored 12 goals in his last 12 games and 21 in all competitions this season, suffered a hamstring injury with France.
Midfield duo Fernando Gago and Pedro Leon are also missing through injury, while Raul Albiol and Sergio Canales have been omitted by Mourinho.
Spurs (from): Gomes, Pletikosa, Cudicini, Bassong, Gallas, Assou-Ekotto, Dawson, Corluka, Bale, Sandro, Jenas, Kranjcar, Lennon, Rose, Huddlestone, Modric, Crouch, Pavlyuchenko, Defoe, Van der Vaart.