Barnsley defenders Bobby Hassell and Jay McEveley have been ruled out of the home game with Burnley.
Hassell was forced out of Saturday's defeat at Portsmouth with a recurrence of his groin injury and McEveley hobbled off late on with an unspecified problem, leaving the Tykes to finish the game with 10 men.
Manager Keith Hill told reporters: "We really are down to the bare bones and it's probably a worse situation than we were in a week ago."
Hill is still without midfielders David Perkins, Matt Done (both hamstring), Reuben Noble-Lazarus (ankle) and Goran Lovre (knee), striker Danny Haynes (hamstring) and defender Luke Potter (knee).
But defender Rob Edwards is expected to play some part after his long-term hamstring injury and striker Craig Davies, used as a late substitute in his first match since late August at Portsmouth following a groin problem, could play a bigger role for Hill's side.
Burnley manager Eddie Howe will pick from the same squad for the visit to Barnsley.
The Clarets were beaten in the ninth minute of injury time of Saturday's clash with Reading at Turf Moor and Howe will give the same players a chance to bounce back.
Winger Keith Treacy will be hoping to earn a start after coming off the bench to play the final 35 minutes against the Royals and he will battle with Junior Stanislas for a start.
Striker Martin Paterson and defender Michael Duff have returned to training following respective thigh and knee injuries, but they are not yet in a position to be considered for selection.
The road to recovery is a longer one for goalkeeper Brian Jensen (groin) and attacker Danny Ings (knee), though, and they are still some way away from fitness.