Yeovil will continue to be without suspended captain Paul Huntington.
Huntington serves the third match of a four-game ban for the visit of the U's, and recent signing Richard Hinds is set to continue to fill in for the Glovers skipper in the heart of defence.
Manager Gary Johnson will be unable to call on full-back Joe Edwards, who has returned to his parent club Bristol City for the time being because of injury, but otherwise Yeovil have a full bill of fitness as they look to build on the 3-2 win at Wycombe in midweek.
On-loan Reading midfielder Lawson D'Ath will be hoping to keep his place in Johnson's starting XI after making his full debut for the Somerset side at Adams Park.
Freddie Sears will go straight into the Colchester squad, subject to paperwork clearance, after joining on loan from West Ham.
Steven Gillespie is likely to have played his way into the starting line-up. The striker scored the U's only goals in their games at Scunthorpe and Chesterfield only to be dropped for the 1-0 win at Leyton Orient prior to the recent cold snap.
Gillespie was again named among the substitutes for Tuesday's clash with Brentford but responded in the best way possible by stepping off the bench to bag a late brace and clinch a 2-1 triumph, staking his obvious claim for a recall.
Elsewhere, manager John Ward is not expected to have any new injury or suspension problems.
Winger Ashley Vincent (hamstring) and defender Matt Heath (groin) continue to increase their fitness work and push for a place in United's matchday squad having both returned to full training.
On-loan forward Gavin Massey has been recalled by parent club Watford and will not get the chance to line up against the Glovers, with whom he spent the first half of the season and duly bagged a brace in a 2-2 draw with Colchester in October's reverse fixture.