Yeovil will be without suspended duo Michael Woods and Ed Upson.
Woods was sent off after 33 minutes of the Glovers' 6-0 home humiliation by Stevenage on April 14 for a rash tackle on Boro's Michael Bostwick and serves the second match of the a three-game ban.
Upson, meanwhile, sits out the final game of his three-match suspension having accrued 15 yellow cards over the course of the campaign.
Otherwise, manager Gary Johnson has a fully-fit squad to choose from. Yeovil secured their League One safety with a 2-2 draw at Leyton Orient last time out but could send Chesterfield down to League Two with either a draw or a win at Huish Park.
Defenders Liam Ridehalgh and Simon Ford are concerns for Chesterfield ahead of another must-win match.
Full-back Ridehalgh was struggling with a knock towards the end of the 2-1 home victory over Rochdale last weekend.
Ford was forced off in the same game after 33 minutes with a cut above his eye but manager John Sheridan is hopeful the centre-half will be ok.
Midfielder Danny Whitaker, scorer of crucial goals from the bench in the last two matches, is a hot tip to start.
Defender Neal Trotman (knee) and veteran striker Jack Lester (groin) remain on the sidelines.
Chesterfield face a fourth straight game knowing that anything but a victory will see them relegated. However, a win on Saturday may also not be enough to prevent an immediate return to League Two if results elsewhere go against them.