Blackpool boss Ian Holloway is set to ring the changes.
Seasiders trio Stephen Crainey, Craig Cathcart and Neal Eardley have been called up for international duty while keeper Matthew Gilks has suffered an ankle injury while training with Scotland and will be unavailable.
Holloway will be giving his squad players a chance to impress against the League One side, with Tom Ince set to make his debut.
Blackpool will be hoping to build on their opening-day win at Hull by reaching the second round for the third consecutive season.
The last time they fell at the first hurdle was in 2008 against Macclesfield and the Bloomfield Road club will be determined to avoid losing to lower league opposition again.
Sheffield Wednesday manager Gary Megson is also likely to ring the changes.
Megson could field an entirely different starting XI to the team that recorded a 2-0 opening-day victory over Rochdale at the weekend.
Wednesday have no fresh injury concerns or suspensions but they face Bournemouth away in the league on Saturday and the strain of two games in three days will force Megson's hand.
New signing Chris O'Grady, who made a cameo appearance from the bench for Rochdale at the weekend, could be in line for his Owls debut.