Charlton boss Chris Powell is set to make wholesale changes.
Powell fielded an entirely new line-up against Reading in the previous round and was rewarded with a fine 2-1 win.
Winger Danny Green could return following a recent illness and defender Leon Cort is pushing to make his full debut having recently joined on loan from Burnley.
Simon Francis, Gary Doherty, Yado Mambo, Ruben Bover, Andy Hughes, Bradley Pritchard and Jason Euell will all be in the frame for starts.
But striker Paul Benson remains a doubt with a broken finger.
Powell said: "There will be one or two changes for Preston."
Preston boss Phil Brown could make several changes to the side that beat Yeovil 4-3 on Friday night.
Club captain Ian Ashbee is set to make his first start of the season.
Ashbee was a late substitute in Friday's game and is a certain starter after recovering from the knee injury that sidelined him for the first six matches of the season.
Veteran midfielder Graham Alexander could be given the night off. The 39-year-old has played in all of North End's games since returning to the club at the beginning of August.
Steven Smith has been struggling with a calf injury and is unlikely to be risked.