Wolves will be without Roger Johnson for the game after he suffered a calf injury during Saturday's 3-0 home defeat by QPR.
Kevin Foley, an unused substitute in that match, is also ruled out with an ankle problem.
Mick McCarthy is set to make several changes, with Adlene Guedioura, Nenad Milijas and David Edwards in contention to start.
Millwall will be without Darius Henderson, Jay Simpson and Liam Feeney.
Henderson suffered a calf injury in training on Thursday which ruled him out of Saturday's goalless draw against West Ham.
Fellow striker Simpson and winger Feeney are cup-tied having already played in the competition for their previous clubs.
Young frontman John Marquis is due to have a hernia operation soon but has been playing on regardless as the problem is not getting any worse, although he may be rested.
Northern Ireland forward Josh McQuoid has missed out on the last two squads but could get a chance at Molineux.
Midfielder Therry Racon (ankle) and striker Shaun Batt (calf) are still out.
Wolves (from): Hennessey, Stearman, Berra, Elokobi, Kightly, Edwards, Henry, O'Hara, Jarvis, Ward, Doyle, Guedioura, Hunt, De Vries, Hammill, Milijas, Ford, Doherty, Vokes.