Jim Bentley will welcome Will Haining and Jason Price back into his Morecambe squad.
Shrimps skipper Haining and striker Price have both been sidelined with hamstring injuries but are fit to face the Owls.
Morecambe's only remaining fitness concern is Laurence Wilson, with the defender again expected to miss out due to a groin complaint.
Meanwhile, Bentley has urged the Globe Arena faithful to inspire his side on what is sure to be a memorable afternoon on the Lancashire coast.
"Whatever happens this is a great occasion for the club and I want everyone to celebrate it," he told The Visitor.
"I really want the fans to get behind us and make a big noise. I would really like to see a wall of red on the terraces and as many flags as possible around the ground with everyone out for an enjoyable day."
Sheffield Wednesday are expecting to be without three of their loanees.
Winger Ben Marshall (Stoke), goalkeeper Stephen Bywater (Derby) and central defender Danny Batth (Wolves) are unlikely to be given permission to play in the first round by their parent clubs.
David Prutton could return to contention as he has shaken off a thigh strain, but fellow midfielder Chris Sedgwick is also hoping to step in if Marshall sits it out.
Richard O'Donnell is poised to stand in for Bywater and manager Gary Megson has plenty of options at centre-half in Batth's absence, with fit-again Reda Johnson (groin), Mark Reynolds and Lewis Buxton all capable of slotting in alongside skipper Rob Jones.
The Owls will also be boosted by the return from suspension of striker Gary Madine and Megson must decide whether to recall him to the starting line-up or stick with Chris O'Grady alongside Ryan Lowe up front.