Roberto Di Matteo has confirmed that David Luiz would miss both legs against the reigning European champions.
The interim Blues boss hoped the defender would recover from his hamstring injury within two weeks, which would mean missing up to four games.
Branislav Ivanovic is available during his domestic three-match suspension.
Gary Cahill (foot) and John Terry (ribs) are both expected to be passed fit, while Fernando Torres will hope for a recall against his compatriots.
Chelsea (from): Cech, Ivanovic, Bosingwa, Terry, Cahill, Cole, Mikel, Meireles, Lampard, Ramires, Mata, Malouda, Drogba, Torres, Sturridge, Kalou, Turnbull.
