Chelsea will assess Didier Drogba on Monday night before deciding on their starting XI for Tuesday night's clash.
Drogba missed Saturday's Premier League game at Arsenal with a knee injury but travelled with the squad to Spain on Monday and was out training at the Nou Camp on Monday evening.
The 34-year-old scored the winner in Wednesday's first leg with the Blues' only shot on target.
He also made a huge contribution in what was a smash-and-grab 1-0 victory, although his theatrical reaction to some of the challenges made on him did not go down well with Barca or many of those who watched the match.
Fernando Torres had another game to forget at the Emirates Stadium on Saturday, making it even more imperative Drogba is fit for Tuesday night.
Defender David Luiz also made the trip in what was a 25-man party, despite appearing to have been ruled out for at least another week with a hamstring injury.
Caretaker manager Roberto Di Matteo is expected to provide a full update on the Ivory Coast star's fitness at his pre-match press conference on Monday evening.
Barcelona forward Lionel Messi was present at training on Monday after missing Sunday's session with what Barca described as "slight physical discomfort".
Chelsea (from): Cech, Ivanovic, Bosingwa, Terry, Cahill, Cole, Bertrand, Mikel, Essien, Meireles, Lampard, Ramires, Mata, Malouda, Drogba, Torres, Sturridge, Kalou, Turnbull.