Cheltenham will welcome back Chris Zebroski.
Zebroski has fully recovered from the illness that kept him out of the defeat to Southend on Saturday and assistant manager Neil Howarth predicts no new injury problems ahead of the midweek match.
"I'm pretty certain that everyone who was available at the weekend will be available," he told the club's official website.
"Chris Zebroski came in and trained and we are quite well physically."
However, the Robins assistant believes the game will come too soon for defender Alan Bennett.
He added: "Alan is a very fit lad. He wins the fitness tests, he looks after himself and he is a good pro but he has had a few setbacks in the last three or four weeks and it may come too early for him but he would love to play."
James Constable is available for Oxford's trip to Cheltenham after serving a one-match suspension.
The forward was sent off for two yellow cards against Exeter and as a result missed Saturday's 4-0 humbling at the hands of Burton.
Manager Chris Wilder described the defeat by the Brewers as his "most disappointing afternoon in football", and could respond with wholesale changes as the U's attempt to avoid a fourth straight defeat.
Luke O'Brien, 24, is in line for a place in the starting XI as the Oxford boss looks to sure up a defence that has shipped 11 goals in the previous three matches.
However, Wilder's options are somewhat limited through a lengthy injury list with Lee Cox, Michael Duberry, Liam Davis, Andy Whing and Harry Worley all ruled out.