York will be without injured defender Clarke Carlisle.
The 32-year-old PFA chairman, a free summer signing after leaving Burnley at the end of last season, was carried off with an ankle injury during the first half of the midweek defeat at Burton and replaced by Chris Doig.
Doig is likely to step in on Saturday, while on-loan Leeds defender Charlie Taylor is pushing for a recall after being left out of the squad altogether on Tuesday night. Jamal Fyfield was recalled to the left-back slot.
Skipper Scott Kerr is edging closer to his first appearance since sustaining knee ligament damage in March, but remains three or four weeks away.
Manager Gary Mills has no other new injury or suspension problems and is not expected to make any unforced changes despite seeing his side slip to their first defeat in six league matches in midweek.
Cheltenham boss Mark Yates has a fully-fit squad to choose from.
Club captain Alan Bennett came through Tuesday night's win over Oxford unscathed following a recent calf injury and looks set to keep his place in defence.
Striker Kaid Mohamed is pushing for a start after coming on as a second-half substitute in the 2-1 victory in midweek.
Cheltenham have no new injury concerns and Yates is likely to make very few changes from the team that beat Oxford.
After a mixed start to their season, the Robins currently sit 10th in League Two, just two points above Saturday's opponents.
Town's only away win of the season came against Aldershot in August and Yates will be looking to add another to their tally in York.