Leam Richardson will begin his second spell in caretaker charge of Accrington following Paul Cook's departure to Chesterfield.
Richardson stepped into the breach when John Coleman left the Crown Ground to move to Rochdale last season and Cook's unveiling as the new Spireites boss on Friday leaves him in the same position.
The former Stanley defender took training on Thursday as Cook agreed personal terms with his new employers.
Richardson has no new injury worries, with midfielder Charlie Barnett the only absentee as he steps up rehabilitation from an ankle problem.
The Reds were beaten 5-1 by Rovers at the end of last season - a defeat fresh in the mind of striker Padraig Amond.
He said: "We kind of owe them one, but it's not a game for revenge or anything like that. We want to get ourselves back on track."
Bristol Rovers' defensive crisis has deepened with Tom Parkes and Adam Virgo ruled out and Garry Kenneth also a doubt.
Parkes will serve a suspension for his red card at AFC Wimbledon, while former Celtic defender Virgo, who also played as a striker on Tuesday, has a knee injury.
Kenneth limped off at the end of that 3-1 defeat with a knock on his knee and manager Mark McGhee will be hoping his fellow Scot does not need to join defenders Danny Woodards, Cian Bolger and Michael Smith on the ever-growing injury list.
Scott Bevan, Matt Gill, Matt Harrold, Shaquille Hunter and Derek Riordan are also unavailable. Winger Fabian Broghammer could return after concussion.