Chesterfield will be without Tendayi Darikwa.
Darikwa was substituted early in the second half for the second game running last weekend when he limped out of the 1-1 draw at Rochdale.
The winger has since seen a specialist and been told he requires a hernia operation that will keep him out for around a month.
Some good news for manager Paul Cook could see the return of Drew Talbot.
The versatile Talbot has been sidelined for a month because of a broken toe. Despite not kicking a ball since suffering the injury in the defeat at Wycombe on January 5, Cook has said Talbot will be recalled if he can prove his fitness.
Jonathan Meades returns to the AFC Wimbledon squad.
The on-loan Bournemouth defender missed Tuesday night's 3-0 defeat at Port Vale as he was on international duty with Wales Under-21s.
But midfielder Harry Pell is still missing as he completes a two-match ban for collecting a 10th yellow card of the season on his Dons debut against Burton last Saturday.
Will Antwi remains a doubt with a hip injury but fellow defender Curtis Osano is fit after a calf problem and was on the bench in midweek.
Boss Neal Ardley played five at the back against Vale but admitted afterwards the tactic backfired, so Pim Balkestein may drop out assuming the Dons revert to a flat back four.