Sheffield United are expected to include new loan signing Dominic Poleon in their squad.
The 19-year-old Leeds striker, who will stay at Bramall Lane until the end of the season, is likely to be named among the substitutes.
Fellow forward Jonathan Forte, on loan from Southampton, scored his first goal since returning to the club in the midweek win at Bury and although he was withdrawn as a precaution after taking a knock, he is expected to be fit to resume up front alongside Dave Kitson.
Manager Danny Wilson, who has no new injury or suspension problems, could name an unchanged starting line-up after his side secured a second successive away win on Tuesday night.
Defender Matt Hill is fit again after recovering from a broken jaw and is pushing for a recall, while deadline-day signing Barry Robson is hoping to make his first start following three substitute appearances.
The win at Bury lifted the Blades to within three points of second-placed Doncaster.
Resurgent Colchester expect Brian Wilson to be fit.
The right-back was forced off just before the hour mark in last weekend's 1-0 win over Preston nursing a calf complaint but has received treatment this week and is expected to line up at Bramall Lane.
Back-up goalkeeper Mark Cousins missed the game due to a wrist problem and he is likely to be out for a couple of weeks, with Shaun Phillips again set to be on the bench behind first-choice Sam Walker.
Captain Kem Izzet, meanwhile, is around a week away from making his return from an ankle injury.
U's boss Joe Dunne has kept faith with an unchanged side for the last three games and is expected to do so again should Wilson prove his fitness. However, left-back Bradley Garmston could tempt him into a change having arrived on an initial month-long loan deal from West Brom on Thursday.
The Essex club have won three of their last four following a nine-game losing streak.