The Robins are yet to taste defeat on home soil since head coach Sean O'Driscoll took charge but their failure to secure a victory on the road has left them six points adrift of safety with seven games remaining.
The likes of Albert Adomah and Liam Kelly are pushing for recalls while Paul Anderson came through 90 minutes following his return to the starting line-up after injury.
But O'Driscoll is limited when it comes to attacking options with striker Ryan Taylor (shoulder) back in training but not yet available, and fellow forward Jon Stead (groin) still sidelined.
City's latest defeat on their travels, a 3-0 loss at Derby on Friday, means a reverse to the Owls would leave the Robins staring at a return to League One after six seasons on football's second rung.
Sheffield Wednesday, meanwhile, will be without suspended defender Lewis Buxton for the game.
Buxton was sent off in Saturday's derby win over Barnsley for a second yellow card offence and will sit out a one-game ban, so Martin Taylor will come into contention for a recall.
Seyi Olofinjana and fellow midfielder Stuart Holden made their debuts after joining on loan last week from Hull and Bolton respectively, and manager Dave Jones must decide if both are capable of playing two games in four days.
Giles Coke, fit again after a foot injury, replaced Holden in the second half on Saturday, while Rhys McCabe, Kieran Lee, and David Prutton provide Jones with extra options in midfield.
Jermaine Johnson is in contention to start. He returned to the bench following international duty with Jamaica, but fellow winger Michail Antonio (groin) is out for the rest of the season.